p21 endogenous protein levels (Santa Cruz Biotechnology)
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Santa Cruz Biotechnology
p21 endogenous protein levels
P21 Endogenous Protein Levels, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 5645 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p21+protein+levels/p21/pmc04225545-103-19-39
Average 96 stars, based on 5645 article reviews
P21 Endogenous Protein Levels, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 5645 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p21+protein+levels/p21/pmc04225545-103-19-39
Average 96 stars, based on 5645 article reviews
p21 endogenous protein levels - by Bioz Stars,
2026-09
96/100 stars
Images
1) Product Images from "Identification of new p53 target microRNAs by bioinformatics and functional analysis"
Article Title: Identification of new p53 target microRNAs by bioinformatics and functional analysis
Journal: BMC Cancer
doi: 10.1186/1471-2407-13-552
Figure Legend Snippet: p53 can bind chromatin region surrounding the identified p53 REs in miR genes. A) ChIP assays were performed in HCT116 p53 +/+ (gray bars) and HCT116 p53 −/− (black bars) upon doxorubicin treatment for 24 hours. The results of Real-Time qPCR are presented as fold of mock treatment, normalized with respect to the signal obtained with Input DNA. The results from three control locations corresponding to promoter regions of Actin, GAPDH and exon 9 of CCNB1 genes were averaged and are also presented in panel A (Neg. Ctrls). P21 and miR-34a occupancy were measured as positive controls. Bars represent average and standard deviations of three independent experiments. C) ChIP assays of MCF7 cells treated with doxorubicin for 24 hours. Results obtained after ChIP with an antibody against IgG were included as a negative control for the p53 miR-REs. Examples of agarose gel analysis of standard ChIP-PCR are given in panels B and D . Specifically, panel B shows experiments performed in HCT116 p53 +/+ and p53 −/− cells, while panel D presents results from MCF7 cells. The DO-1 p53 antibody was used for immunoprecipitation; NTC, no template control. Regions surrounding the established P21 and miR-34a p53 REs were examined as positive controls.
Techniques Used: Control, Negative Control, Agarose Gel Electrophoresis, Immunoprecipitation
Figure Legend Snippet: p53-induced expression of mature and pre-miR genes. RT-qPCR were performed in HCT116 p53 +/+ (black bars), HCT116 p53 −/− (white bars) and MCF7 (gray bars) cells upon doxorubicin (A, B) or Nutlin (C) for 24 hours. The expression of the processed mature miR (A) or of the pre-miR RNA (B, C) was tested. p21 mRNA expression was measured as p53-dependent positive control. PVT1 non-coding RNA expression levels were measured as additional evidence of p53-dependent expression of miR-1204 and miR-1206. Results are presented as fold of induction with respect to the mock condition. Bars plot average and standard deviations of three independent experiments. (D) Western Blot establishing stabilization of p53 protein in doxorubicin and Nutlin treated cells and the induction of the p53 target gene p21. GAPDH was used as reference.
Techniques Used: Expressing, Quantitative RT-PCR, Positive Control, RNA Expression, Western Blot
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